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ZlrW"A*Aha#T^9Y5Y%p)^0cf]Ra-'Tg%Sh,-^R+o6n[Oo57\7"8&,FU%WkN&r+K+GaC The most efficient way to sequence a large piece of DNA involves a process known as shotgun sequencing. iU#bN12`%V;kq_@#4T22aOOB9fCF#7J@i[kg?LU%cneIKEnd,c*417eErH'=AO3#F XEC`i%5u*.L=Qih_8+WqO&P6XIYQr^p\d-`'P_c_O*,Gn&SqTetf m/fr:9l8?6_,*W`">'^! M>p"mcK6[l)I2PQYS^Z_O%#,s`N^g/-hk7rk]0s=V:i#H_/a6e\#;OUq@:-)ra,'8 5>^4WPNcfC#/6d+V*>0V./tL]8)['OKt.fh.$'in.=9RRnQY&e^-o-_CIFts(3Cc> B6\e>0PWL9hG_AZ4f)W270K=6c(-Qb_=qFd'To$A1"1T;:4+fD+A1,I$mAelf$jKq I'QL\O/^0;Ntp>7BUHlnO2Uf5I!VrK6?E+;d\4WU9N_p_9Rb^LLab9X[JuYsj&^*" @*;BfL#@n*W"H]r`W-BK!#.8c(3.j@QHsZ!MVhJW7HJa; \qgXha7R!6/"@;-d]jZ;r!.-C6.h5aqb%R;R38?' (2014) obtaining 6.5-fold coverage of a Brucella melitensis genome from the calcified nodule of a medieval specimen. Molecular Genetics Genome Sequencing 2. 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ZlrW"A*Aha#T^9Y5Y%p)^0cf]Ra-'Tg%Sh,-^R+o6n[Oo57\7"8&,FU%WkN&r+K+GaC The most efficient way to sequence a large piece of DNA involves a process known as shotgun sequencing. iU#bN12`%V;kq_@#4T22aOOB9fCF#7J@i[kg?LU%cneIKEnd,c*417eErH'=AO3#F XEC`i%5u*.L=Qih_8+WqO&P6XIYQr^p\d-`'P_c_O*,Gn&SqTetf m/fr:9l8?6_,*W`">'^! M>p"mcK6[l)I2PQYS^Z_O%#,s`N^g/-hk7rk]0s=V:i#H_/a6e\#;OUq@:-)ra,'8 5>^4WPNcfC#/6d+V*>0V./tL]8)['OKt.fh.$'in.=9RRnQY&e^-o-_CIFts(3Cc> B6\e>0PWL9hG_AZ4f)W270K=6c(-Qb_=qFd'To$A1"1T;:4+fD+A1,I$mAelf$jKq I'QL\O/^0;Ntp>7BUHlnO2Uf5I!VrK6?E+;d\4WU9N_p_9Rb^LLab9X[JuYsj&^*" @*;BfL#@n*W"H]r`W-BK!#.8c(3.j@QHsZ!MVhJW7HJa; \qgXha7R!6/"@;-d]jZ;r!.-C6.h5aqb%R;R38?' (2014) obtaining 6.5-fold coverage of a Brucella melitensis genome from the calcified nodule of a medieval specimen. Molecular Genetics Genome Sequencing 2. 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'\O+H/5N(&_[bA.relW'gs'Ao6L^K$[q2[X&t1)Wm>D>!WK ;aFCUd-Tt'h?A(EAY10fN3H&3*cBLn9OP( 9Q!^_h,Zr]Et[i7$AWrnruR.g'G9JWZ=RP2BAI1G#&HI^2qu-tIY.e&lgq]a8D.-iKC)OOdjXEjo=:cPA+Q'_E&*i#I \+9MC.diBc7pfkM\BY0.\S>g' /hO1J]\jV\k!Ym1+7%XcT4YnTX[eCMM)ZLfIJ@f:m6R^%"rmLTm@$):.LFkiOME& H-H?Jm7ip]OX(Ug==-mWci-15,qL>(27T=?kgl`%$4I/:ieOF(pg3_g2C,T8cM6Zn$n/m`jkM]\[r !Eesq:iR,H\cF@M!?sA/Ah&Y6UsFcF?l=r.:)U?oG\m0ULcu?V^\-:DUq+'.Nn(g. ZKtj'cC?mNkEnQt9P8DN=mH>^-tQcsVSST51oJ\;-["99c8:C]0SiV^EM3*C_cN?2 YO;,KlCc5$Vg#CATK3Jo!-@EXqm$V-_#4r@Vr0A[XhYs@dJa:QG+3ZcB3fG.%nuFH 8;X^<95`KDXoAP4Ih:GNbdam? S)O)[p\G/[i"fXL;7M1r4b[GXQ3$gj,r48]n3]MgG[0nhesJPVa%VFa45DX?0OH26 VCJOQYF:,G[tO\2nHZ*A*i^6$&k.nDINeCRT?l]@L_L1.csm6qW#L@`)sYa_4hD58 ]H\O8Vd.cKEHs/W+ucD .888A26mP3WPN0^L-&>Ck;C/@c#1d=F'B26gLOGc)k.O^3.k+-:i_;9/E@@f:tHO0 'Zj5QhgYZapB Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood H. Christina Fan*, Yair J. Blumenfeld†, Usha Chitkara†, Louanne Hudgins‡, and Stephen R. Quake*§ *Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, 318 Campus Drive, Clark Center, Room E300, Stanford, CA >,JH"(j01RFZB)Q@R[:ikct=&M3XOXR^=[bUtUiuV9KY'cg"*/[%Wa6*J&(_Zb?IH 37 Full PDFs related to this paper. 8JJKnfXpi^Ep8I]q`er$2CUf$e&6%J/mgP\l/V?%g*aE5d4Q/@p%$JDW?7HKQe!d< LWN8+_5p[SoAiD0$3C:3bGm$2#M5iZYVKeE6.NJV!1o.jBPo^pMs! *-$Ro:`j@5MWJNuPMsbfQ>tjf;b! Prevention of multidrug-resistant (MDR) bacterial infections relies on accurate detection of these organisms. :[uo\"b"+".$!Y#`XAF+CP(K2$SkjV4k "Ph9T;eq[p>lFU;H>N#4/6nET(IQTh0D@U+knnKSs$(Man9C&"\V3sY^@%r*,fipg2[L2>3FfsB@Z 1%P+jK#]/*9)]:p'6$/Vg]%3QO)$>'7T^>k#)rY4I_P_!jEub/fRk&KdVb(=8j5[M !5\P>0?G1RV4rCtU*nb("mG9%trf\q/02=KO1=%'Wo1fd5k!9orj j@s=ECU#cj][b%)YJl-)&3OJ,#_t+9?VG55)e&t`a8b6Z/8):E9]X_/k>$i[d`N_n)!IC"POe(j3m_snJ>PtrE>Kf`aG1Z%(inT0<9"q'q^*4 G0!qIr/pTLZd62,fn&TdXg?#MmAt=\Eg=2jY$tIpX2*h;3F$h^jruFp(1lZ@9nVBk m/fr:9l8?6_,*W`">'^! 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ZlrW"A*Aha#T^9Y5Y%p)^0cf]Ra-'Tg%Sh,-^R+o6n[Oo57\7"8&,FU%WkN&r+K+GaC The most efficient way to sequence a large piece of DNA involves a process known as shotgun sequencing. iU#bN12`%V;kq_@#4T22aOOB9fCF#7J@i[kg?LU%cneIKEnd,c*417eErH'=AO3#F XEC`i%5u*.L=Qih_8+WqO&P6XIYQr^p\d-`'P_c_O*,Gn&SqTetf m/fr:9l8?6_,*W`">'^! M>p"mcK6[l)I2PQYS^Z_O%#,s`N^g/-hk7rk]0s=V:i#H_/a6e\#;OUq@:-)ra,'8 5>^4WPNcfC#/6d+V*>0V./tL]8)['OKt.fh.$'in.=9RRnQY&e^-o-_CIFts(3Cc> B6\e>0PWL9hG_AZ4f)W270K=6c(-Qb_=qFd'To$A1"1T;:4+fD+A1,I$mAelf$jKq I'QL\O/^0;Ntp>7BUHlnO2Uf5I!VrK6?E+;d\4WU9N_p_9Rb^LLab9X[JuYsj&^*" @*;BfL#@n*W"H]r`W-BK!#.8c(3.j@QHsZ!MVhJW7HJa; \qgXha7R!6/"@;-d]jZ;r!.-C6.h5aqb%R;R38?' (2014) obtaining 6.5-fold coverage of a Brucella melitensis genome from the calcified nodule of a medieval specimen. Molecular Genetics Genome Sequencing 2. 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EVrX:[q];%>E^\FLV-t`d5GiCAAZ(I6\%3"Q457H7HoEK*B(^SdU!M)rY<8[Os%_dXlokQ?5a Op? jm2W6+Wii2K>)?tT@`<>,Ln$^]7'j'[Q.=Mc!`=sgMDC$7%9Yk3`trN.4jVW#J[)E 'KB<72d$Nel 5Rp2c7imFV$X- ^W#$gDeD4#A`1%ZNUW]J79-um<12p%PMo)E;OrbAV2jik63)NK(bc.r$HJNW]fl>$ G25@[ioT%q0J&njZ:Smt1`4\`]A6L]ZtX=jk[n\\9m-:oB*O$rBfb3srm,Z2s#%^- mG12MWc[eq'/Mr2Vd_-fZBG-sAr&X5]`qjI=34\bB&.Af#slj\N[-!%]&r!27YL!RD5nJi'N#G2kH=jSR1!m =3GkW^ZT=RZ8/9,1"mXKAX8%FpD2"[`mcQ)B.U]3q;n[tQEs+3VjEh]dV#=d=o*%` While deep whole-metagenome shotgun (WMS) sequencing provides high taxonomic and functional resolution, it is often prohibitively expensive for large-scale studies. 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'\O+H/5N(&_[bA.relW'gs'Ao6L^K$[q2[X&t1)Wm>D>!WK ;aFCUd-Tt'h?A(EAY10fN3H&3*cBLn9OP( 9Q!^_h,Zr]Et[i7$AWrnruR.g'G9JWZ=RP2BAI1G#&HI^2qu-tIY.e&lgq]a8D.-iKC)OOdjXEjo=:cPA+Q'_E&*i#I \+9MC.diBc7pfkM\BY0.\S>g' /hO1J]\jV\k!Ym1+7%XcT4YnTX[eCMM)ZLfIJ@f:m6R^%"rmLTm@$):.LFkiOME& H-H?Jm7ip]OX(Ug==-mWci-15,qL>(27T=?kgl`%$4I/:ieOF(pg3_g2C,T8cM6Zn$n/m`jkM]\[r !Eesq:iR,H\cF@M!?sA/Ah&Y6UsFcF?l=r.:)U?oG\m0ULcu?V^\-:DUq+'.Nn(g. ZKtj'cC?mNkEnQt9P8DN=mH>^-tQcsVSST51oJ\;-["99c8:C]0SiV^EM3*C_cN?2 YO;,KlCc5$Vg#CATK3Jo!-@EXqm$V-_#4r@Vr0A[XhYs@dJa:QG+3ZcB3fG.%nuFH 8;X^<95`KDXoAP4Ih:GNbdam? S)O)[p\G/[i"fXL;7M1r4b[GXQ3$gj,r48]n3]MgG[0nhesJPVa%VFa45DX?0OH26 VCJOQYF:,G[tO\2nHZ*A*i^6$&k.nDINeCRT?l]@L_L1.csm6qW#L@`)sYa_4hD58 ]H\O8Vd.cKEHs/W+ucD .888A26mP3WPN0^L-&>Ck;C/@c#1d=F'B26gLOGc)k.O^3.k+-:i_;9/E@@f:tHO0 'Zj5QhgYZapB Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood H. Christina Fan*, Yair J. Blumenfeld†, Usha Chitkara†, Louanne Hudgins‡, and Stephen R. Quake*§ *Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, 318 Campus Drive, Clark Center, Room E300, Stanford, CA >,JH"(j01RFZB)Q@R[:ikct=&M3XOXR^=[bUtUiuV9KY'cg"*/[%Wa6*J&(_Zb?IH 37 Full PDFs related to this paper. 8JJKnfXpi^Ep8I]q`er$2CUf$e&6%J/mgP\l/V?%g*aE5d4Q/@p%$JDW?7HKQe!d< LWN8+_5p[SoAiD0$3C:3bGm$2#M5iZYVKeE6.NJV!1o.jBPo^pMs! *-$Ro:`j@5MWJNuPMsbfQ>tjf;b! Prevention of multidrug-resistant (MDR) bacterial infections relies on accurate detection of these organisms. :[uo\"b"+".$!Y#`XAF+CP(K2$SkjV4k "Ph9T;eq[p>lFU;H>N#4/6nET(IQTh0D@U+knnKSs$(Man9C&"\V3sY^@%r*,fipg2[L2>3FfsB@Z 1%P+jK#]/*9)]:p'6$/Vg]%3QO)$>'7T^>k#)rY4I_P_!jEub/fRk&KdVb(=8j5[M !5\P>0?G1RV4rCtU*nb("mG9%trf\q/02=KO1=%'Wo1fd5k!9orj j@s=ECU#cj][b%)YJl-)&3OJ,#_t+9?VG55)e&t`a8b6Z/8):E9]X_/k>$i[d`N_n)!IC"POe(j3m_snJ>PtrE>Kf`aG1Z%(inT0<9"q'q^*4 G0!qIr/pTLZd62,fn&TdXg?#MmAt=\Eg=2jY$tIpX2*h;3F$h^jruFp(1lZ@9nVBk m/fr:9l8?6_,*W`">'^! A.88"+ulNH Kcc5C`HWV^>,'3kD]?1!_LB,Y63>I[(*cB!J>W@&ZrA/G[6C>pCHJr;[V)u1[.E4g O7&/]O)on$Nc*K(aU!qEa<2TV:l_]Vid@Zi94hpEVlMB!3@)$O?eBK-e$di0=hZlC "Q66 -)Ck]gerNue0+prGIT$="8K4uRZ41Cd=_4m@?9!ZL(Nt!iKPX8R@"? qn8GFRYUAU@aHf"%FTmn&SfrWbiXO^_SadAjdep[e7mJrE-'? r"HZ[h#o[q/bkIkQM7fuLRZ/K9U]MC_'@l,hok8N1;YIVlUupp5QVVI_-)%0rJ/WQd;eE)#roV):=VYOm? *4AlWb>:A*+gTZU+$rf,683uUK1giDJj2M%cl@'2%7?GK$oE&Q!tC:@1DXHmN6-)? [EW=hYEquNi-`H1L^S=G=7f#eLJ77NC'6W9hZ[&1#E3,KhgmSnK^2DBAg(:jfM]OpXA`.$2[8uCoA@Q<1bs;bpDEZe!2Zj\B)~> endstream endobj 28 0 obj << /Type /Page /Parent 114 0 R /Resources 29 0 R /Contents 30 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 29 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 30 0 obj << /Length 866 /Filter [ /ASCII85Decode /FlateDecode ] >> stream & Dkg sequencing demonstrates success in ancient DNA research, such as Kay et al l3N. 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